I recently discovered the news about MGO. What is MGO? Well it stands for methylglyoxal. This material is actually useful for healing properties. At least that is according to study conducted in the University of Queensland, which mentioned that honey extracted from the native myrtle contains healing properties in the form of methylglyoxal (MGO).
Read more if you want to know more about MGO and/or honey usage benefit for your health.
As we face more challenges when treating and healing humans, revisiting therapies that fell out of favor has become more common. For example, people with open wounds that are not healing receive judicious applications of maggots to remove necrotic tissue and promote healing. Leeches are used for patients after surgery to prevent blood clotting in swollen tissues and encourage healing. However, not all therapies involve direct application of squirmy creatures to skin; in fact, honey is one item people are willing to have in their homes. Honey has been used as a sweetener for food, for soothing sore throats and coughs and, more recently, for treating wounds unresponsive to traditional antibiotic therapy. In a recent PLoS ONE paper, the authors assessed the properties (MGO) that are associated with honey’s antimicrobial activity against pathogenic and food-spoiling bacteria.
Kwakman et al. examined two different medical-grade honeys, unprocessed Revamil® source (RS) honey from the Netherlands and nonsterilized UMF™ 16+ Active manuka honey from the United Kingdom. Using 40% (v/v) as the starting amount, RS and manuka honeys were serially diluted twofold to determine maximal dilution that reduced bacterial survival 1000-fold for methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Pseudomonas aeruginosa, pathogens found in wounds, and Bacillus subtilis, a food-spoiling agent. After two hours, RS honey inhibited growth of all bacteria but MRSA at 2.5- to 13-fold dilutions, but manuka honey only showed activity against B. subtilis at 2.5-fold dilution. After a 24-hour incubation, manuka honey had more potent antimicrobial activity against all tested bacteria (e.g., tenfold dilution of RS honey versus 80-fold dilution of manuka honey to kill MRSA). Each honey demonstrated effective killing at both time points but at different levels against each tested microbe.
The known factors responsible for the bactericidal activity of honey include high sugar concentration, H2O2, the 1,2-dicarbonyl compound methylglyoxal (MGO), the cationic antimicrobial peptide bee defensin-1 and low pH. In 40% (v/v) honey, H2O2 concentration for RS honey was determined to be 3.47 ± 0.25mM after accumulating for 24 hours, while manuka honey had no measurable H2O2. Levels of MGO differed as well: 0.25 ± 0.01mM for RS honey and 10.94 ± 1.70mM for manuka honey. Bee defensin-1 protein levels in honey were determined by concentrating the proteins, separating the samples on native acid-urea polyacrylamide gel electrophoresis (AU-PAGE) and visualized by parallel Coomassie-staining and a B. subtilis growth inhibition overlay assay. Only the RS honey showed any significant staining on the gel and inhibited B. subtilis growth on agarose.
Because the two medical honeys tested by Kwakman et al. differed in their bactericidal activities, manuka honey was further assessed in the 24-hour treatment for factors that contribute to its effectiveness against bacteria. Since manuka honey had much higher MGO levels than RS honey and showed no other activity (H2O2 and bee defensin-1), researchers neutralized MGO in manuka honey and the same panel of bacteria were assessed. Without MGO, the manuka honey activity against MRSA was reduced to that of a honey-equivalent sugar solution. However, E. coli killing activity had not changed and higher dilutions of honey were needed to kill B. subtilis and P. aeruginosa (two- and eightfold more honey, respectively). To examine what cationic factors contributed to manuka honey’s activity, a polyanionic compound that neutralizes cationic compounds was added. As a result, activity against P. aeruginosa was lost while effectiveness again B. subtilis and E. coli was reduced. Even though manuka honey had no measurable H2O2, catalase was added to neutralize H2O2 with no change in antimicrobial activity. Finally, the MGO- and cationic-neutralized honey had its pH adjusted to 7.0 and was tested again. Activity against E. coli was abolished while manuka honey retained activity against B. subtilis albeit at a lower level. The dilution and neutralization tests revealed that honey concentration, MGO and additional cationic and noncationic factors are part of the 24-hour antimicrobial activity of manuka honey.
Which factors contribute to the bacterial killing after only two hours? For RS honey, neutralizing antimicrobial bee defensin-1 peptide increased the amount of honey needed to kill B. subtilis from 5% to 40% but did not change the amount of honey needed to kill E. coli or P. aeruginosa. After also neutralizing H2O2, RS honey was no more effective at killing B. subtilis and E. coli than honey-equivalent sugar solution; activity against P. aeruginosa with at 40% honey was only one order of magnitude above honey-equivalent sugar solution. Any remaining activity against P. aeruginosa was destroyed when the pH was increased to 7. For manuka honey, two-hour activity against B. subtilis was reduced when MGO was neutralized. However, the remaining antimicrobial activity was unaffected by adjusting the pH to 7 or adding catalase to destroy H2O2 and was further reduced but not abolished when treated with polyanionic compound.
Before I read this article, I had no idea that honey’s antibacterial activity was multifactorial and heavily concentration-dependent. Since the two honeys examined came from two different sources, different bactericidal factors are not unexpected, but I was surprised that they differed so greatly. Because of the multiple factors that contribute to the antibacterial activity, microbes have difficulty circumventing the killing effects of honey versus that of antibiotic therapy. Now I have a better appreciation that honey can take care of nasty microbes on our bodies and in our food. And if I had a nonhealing wound and was asked to choose between treatment with honey or with maggots, no contest.
Kwakman, P., te Velde, A., de Boer, L., Vandenbroucke-Grauls, C. and Zaat, S. (2011). Two Major Medicinal Honeys Have Different Mechanisms of Bactericidal Activity. PLoS ONE, 6 (3) DOI: 10.1371/journal.pone.0017709
Flavors of honey have always captured the attention of researchers. Going with the same line, a team of researchers from Brisbane unveiled the most effective anti-bacterial medicinal honey. As per a study conducted in the University of Queensland, honey extracted from the native myrtle contains healing properties in the form of methylglyoxal (MGO).
The full fledge research programme undertook regular trails on the native species of myrtle, grown across the Australian eastern seaboard. The researchers found that inclusion of 500 – 1750 mg/kg MGO can be used to combat the bacterial infection of deal with Methicillin-Resistant staphylococcus aureus (MRSA).
Responding to the study, the Chief Executive of a company involved in the research, Carolyn MacGill asserted the beneficial properties of honey to fight against various lethal infections.
Citing the medicinal properties of honey, MacGill claimed that honey can prove to be a best alternative treatment for MRSA and staph-type infections in place of existing treatment from penicillin and penicillin derivatives.
Additionally, probing natural therapies can be used extensively in wide range of treatments in the field of medical science. Confirming the potential benefits of honey, Ms MacGill claimed that the long research work in this field can deliver beneficial results for Australian and international medical fraternities.
Honey sourced from a native myrtle tree in Australia has been hailed as the “world’s most potent” after it’s found to have the strongest anti-bacterial properties in the globe.
A research team has claimed the honey from the myrtle tree could be used to treat antibiotic-resistant bacterial infections that commonly occur in hospitals and nursing homes. According to researchers, the myrtle honey has very high levels of the anti-bacterial compound Methylglyoxal (MGO) and it outperforms all medicinal honeys currently available on the market.
Lead researcher Yasmina Sultanbawa said potency of the honeys meant a small amount was required to fight infection. “The sheer strength, due to high levels of active compounds in these honeys, has meant that we have been able to completely inhibit MRSA for example in in-vitro studies with a small quantity of the honey,” she said.
I hope above posting is informative enough for anybody who looking info about usage of honey and its MGO properties.